Quantitative Proteomic and Phosphoproteomic Approaches for Deciphering the Signaling Pathway for Tension Wood Formation in Poplar.
Identifieur interne : 001B69 ( Main/Exploration ); précédent : 001B68; suivant : 001B70Quantitative Proteomic and Phosphoproteomic Approaches for Deciphering the Signaling Pathway for Tension Wood Formation in Poplar.
Auteurs : Mélanie Mauriat [France] ; Jean-Charles Leplé [France] ; Stéphane Claverol [France] ; Jérôme Bartholomé [France] ; Luc Negroni [France] ; Nicolas Richet [France] ; Céline Lalanne [France] ; Marc Bonneu [France] ; Catherine Coutand [France] ; Christophe Plomion [France]Source :
- Journal of proteome research [ 1535-3907 ] ; 2015.
Descripteurs français
- KwdFr :
- Analyse de regroupements (MeSH), Bois (génétique), Bois (métabolisme), Données de séquences moléculaires (MeSH), Gene Ontology (MeSH), Gravitation (MeSH), Gravitropisme (MeSH), Peptides (génétique), Peptides (métabolisme), Phosphopeptides (génétique), Phosphopeptides (métabolisme), Phosphoprotéines (génétique), Phosphoprotéines (métabolisme), Populus (génétique), Populus (métabolisme), Protéines végétales (classification), Protéines végétales (génétique), Protéines végétales (métabolisme), Protéome (classification), Protéome (génétique), Protéome (métabolisme), Protéomique (méthodes), Régulation de l'expression des gènes végétaux (MeSH), Réseaux de régulation génique (MeSH), Spectrométrie de masse (MeSH), Séquence d'acides aminés (MeSH), Transduction du signal (génétique), Xylème (génétique), Xylème (métabolisme).
- MESH :
- classification : Protéines végétales, Protéome.
- génétique : Bois, Peptides, Phosphopeptides, Phosphoprotéines, Populus, Protéines végétales, Protéome, Transduction du signal, Xylème.
- métabolisme : Bois, Peptides, Phosphopeptides, Phosphoprotéines, Populus, Protéines végétales, Protéome, Xylème.
- méthodes : Protéomique.
- Analyse de regroupements, Données de séquences moléculaires, Gene Ontology, Gravitation, Gravitropisme, Régulation de l'expression des gènes végétaux, Réseaux de régulation génique, Spectrométrie de masse, Séquence d'acides aminés.
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), Cluster Analysis (MeSH), Gene Expression Regulation, Plant (MeSH), Gene Ontology (MeSH), Gene Regulatory Networks (MeSH), Gravitation (MeSH), Gravitropism (MeSH), Mass Spectrometry (MeSH), Molecular Sequence Data (MeSH), Peptides (genetics), Peptides (metabolism), Phosphopeptides (genetics), Phosphopeptides (metabolism), Phosphoproteins (genetics), Phosphoproteins (metabolism), Plant Proteins (classification), Plant Proteins (genetics), Plant Proteins (metabolism), Populus (genetics), Populus (metabolism), Proteome (classification), Proteome (genetics), Proteome (metabolism), Proteomics (methods), Signal Transduction (genetics), Wood (genetics), Wood (metabolism), Xylem (genetics), Xylem (metabolism).
- MESH :
- chemical , classification : Plant Proteins, Proteome.
- chemical , genetics : Peptides, Phosphopeptides, Phosphoproteins, Plant Proteins, Proteome.
- chemical , metabolism : Peptides, Phosphopeptides, Phosphoproteins, Plant Proteins, Proteome.
- genetics : Populus, Signal Transduction, Wood, Xylem.
- metabolism : Populus, Wood, Xylem.
- methods : Proteomics.
- Amino Acid Sequence, Cluster Analysis, Gene Expression Regulation, Plant, Gene Ontology, Gene Regulatory Networks, Gravitation, Gravitropism, Mass Spectrometry, Molecular Sequence Data.
Abstract
Trees adjust their growth following forced changes in orientation to re-establish a vertical position. In angiosperms, this adjustment involves the differential regulation of vascular cambial activity between the lower (opposite wood) and upper (tension wood) sides of the leaning stem. We investigated the molecular mechanisms leading to the formation of differential wood types through a quantitative proteomic and phosphoproteomic analysis on poplar subjected to a gravitropic stimulus. We identified and quantified 675 phosphopeptides, corresponding to 468 phosphoproteins, and 3 763 nonphosphorylated peptides, corresponding to 1 155 proteins, in the differentiating xylem of straight-growing trees (control) and trees subjected to a gravitational stimulus during 8 weeks. About 1% of the peptides were specific to a wood type (straight, opposite, or tension wood). Proteins quantified in more than one type of wood were more numerous: a mixed linear model showed 389 phosphopeptides and 556 proteins to differ in abundance between tension wood and opposite wood. Twenty-one percent of the phosphoproteins identified here were described in their phosphorylated form for the first time. Our analyses revealed remarkable developmental molecular plasticity, with wood type-specific phosphorylation events, and highlighted the involvement of different proteins in the biosynthesis of cell wall components during the formation of the three types of wood.
DOI: 10.1021/acs.jproteome.5b00140
PubMed: 26112267
Affiliations:
- France
- Aquitaine, Auvergne (région administrative), Auvergne-Rhône-Alpes, Centre-Val de Loire, Nouvelle-Aquitaine, Région Centre
- Bordeaux, Cestas, Clermont-Ferrand, Orléans, Pessac
- Université Bordeaux II
Links toward previous steps (curation, corpus...)
Le document en format XML
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Bordeaux, BIOGECO, UMR1202, F-33615 Pessac, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>‡Univ. 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Bordeaux, BIOGECO, UMR1202, F-33615 Pessac, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>‡Univ. Bordeaux, BIOGECO, UMR1202, F-33615 Pessac</wicri:regionArea><placeName><region type="region" nuts="2">Nouvelle-Aquitaine</region><region type="old region" nuts="2">Aquitaine</region><settlement type="city">Pessac</settlement></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2015">2015</date><idno type="RBID">pubmed:26112267</idno><idno type="pmid">26112267</idno><idno type="doi">10.1021/acs.jproteome.5b00140</idno><idno type="wicri:Area/Main/Corpus">001C43</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001C43</idno><idno type="wicri:Area/Main/Curation">001C43</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">001C43</idno><idno type="wicri:Area/Main/Exploration">001C43</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Quantitative Proteomic and Phosphoproteomic Approaches for Deciphering the Signaling Pathway for Tension Wood Formation in Poplar.</title><author><name sortKey="Mauriat, Melanie" sort="Mauriat, Melanie" uniqKey="Mauriat M" first="Mélanie" last="Mauriat">Mélanie Mauriat</name><affiliation wicri:level="3"><nlm:affiliation>†INRA, UMR 1202 BIOGECO, F-33610 Cestas, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>†INRA, UMR 1202 BIOGECO, F-33610 Cestas</wicri:regionArea><placeName><region type="region" nuts="2">Nouvelle-Aquitaine</region><region type="old region" nuts="2">Aquitaine</region><settlement type="city">Cestas</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>‡Univ. Bordeaux, BIOGECO, UMR1202, F-33615 Pessac, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>‡Univ. Bordeaux, BIOGECO, UMR1202, F-33615 Pessac</wicri:regionArea><placeName><region type="region" nuts="2">Nouvelle-Aquitaine</region><region type="old region" nuts="2">Aquitaine</region><settlement type="city">Pessac</settlement></placeName></affiliation></author><author><name sortKey="Leple, Jean Charles" sort="Leple, Jean Charles" uniqKey="Leple J" first="Jean-Charles" last="Leplé">Jean-Charles Leplé</name><affiliation wicri:level="3"><nlm:affiliation>§INRA, UR0588 AGPF, 2163 Avenue de la Pomme de Pin, CS 40001 Ardon, F-45075 Orléans Cedex 2, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>§INRA, UR0588 AGPF, 2163 Avenue de la Pomme de Pin, CS 40001 Ardon, F-45075 Orléans Cedex 2</wicri:regionArea><placeName><region type="region" nuts="2">Centre-Val de Loire</region><region type="old region" nuts="2">Région Centre</region><settlement type="city">Orléans</settlement></placeName></affiliation></author><author><name sortKey="Claverol, Stephane" sort="Claverol, Stephane" uniqKey="Claverol S" first="Stéphane" last="Claverol">Stéphane Claverol</name><affiliation wicri:level="4"><nlm:affiliation>⊥Plateforme Protéome, CGFB, Université Bordeaux Segalen, F-33076 Bordeaux, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>⊥Plateforme Protéome, CGFB, Université Bordeaux Segalen, F-33076 Bordeaux</wicri:regionArea><placeName><region type="region" nuts="2">Nouvelle-Aquitaine</region><region type="old region" nuts="2">Aquitaine</region><settlement type="city">Bordeaux</settlement></placeName><orgName type="university">Université Bordeaux II</orgName></affiliation></author><author><name sortKey="Bartholome, Jerome" sort="Bartholome, Jerome" uniqKey="Bartholome J" first="Jérôme" last="Bartholomé">Jérôme Bartholomé</name><affiliation wicri:level="3"><nlm:affiliation>†INRA, UMR 1202 BIOGECO, F-33610 Cestas, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>†INRA, UMR 1202 BIOGECO, F-33610 Cestas</wicri:regionArea><placeName><region type="region" nuts="2">Nouvelle-Aquitaine</region><region type="old region" nuts="2">Aquitaine</region><settlement type="city">Cestas</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>‡Univ. Bordeaux, BIOGECO, UMR1202, F-33615 Pessac, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>‡Univ. Bordeaux, BIOGECO, UMR1202, F-33615 Pessac</wicri:regionArea><placeName><region type="region" nuts="2">Nouvelle-Aquitaine</region><region type="old region" nuts="2">Aquitaine</region><settlement type="city">Pessac</settlement></placeName></affiliation></author><author><name sortKey="Negroni, Luc" sort="Negroni, Luc" uniqKey="Negroni L" first="Luc" last="Negroni">Luc Negroni</name><affiliation wicri:level="4"><nlm:affiliation>⊥Plateforme Protéome, CGFB, Université Bordeaux Segalen, F-33076 Bordeaux, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>⊥Plateforme Protéome, CGFB, Université Bordeaux Segalen, F-33076 Bordeaux</wicri:regionArea><placeName><region type="region" nuts="2">Nouvelle-Aquitaine</region><region type="old region" nuts="2">Aquitaine</region><settlement type="city">Bordeaux</settlement></placeName><orgName type="university">Université Bordeaux II</orgName></affiliation></author><author><name sortKey="Richet, Nicolas" sort="Richet, Nicolas" uniqKey="Richet N" first="Nicolas" last="Richet">Nicolas Richet</name><affiliation wicri:level="3"><nlm:affiliation>§INRA, UR0588 AGPF, 2163 Avenue de la Pomme de Pin, CS 40001 Ardon, F-45075 Orléans Cedex 2, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>§INRA, UR0588 AGPF, 2163 Avenue de la Pomme de Pin, CS 40001 Ardon, F-45075 Orléans Cedex 2</wicri:regionArea><placeName><region type="region" nuts="2">Centre-Val de Loire</region><region type="old region" nuts="2">Région Centre</region><settlement type="city">Orléans</settlement></placeName></affiliation></author><author><name sortKey="Lalanne, Celine" sort="Lalanne, Celine" uniqKey="Lalanne C" first="Céline" last="Lalanne">Céline Lalanne</name><affiliation wicri:level="3"><nlm:affiliation>†INRA, UMR 1202 BIOGECO, F-33610 Cestas, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>†INRA, UMR 1202 BIOGECO, F-33610 Cestas</wicri:regionArea><placeName><region type="region" nuts="2">Nouvelle-Aquitaine</region><region type="old region" nuts="2">Aquitaine</region><settlement type="city">Cestas</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>‡Univ. Bordeaux, BIOGECO, UMR1202, F-33615 Pessac, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>‡Univ. Bordeaux, BIOGECO, UMR1202, F-33615 Pessac</wicri:regionArea><placeName><region type="region" nuts="2">Nouvelle-Aquitaine</region><region type="old region" nuts="2">Aquitaine</region><settlement type="city">Pessac</settlement></placeName></affiliation></author><author><name sortKey="Bonneu, Marc" sort="Bonneu, Marc" uniqKey="Bonneu M" first="Marc" last="Bonneu">Marc Bonneu</name><affiliation wicri:level="4"><nlm:affiliation>⊥Plateforme Protéome, CGFB, Université Bordeaux Segalen, F-33076 Bordeaux, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>⊥Plateforme Protéome, CGFB, Université Bordeaux Segalen, F-33076 Bordeaux</wicri:regionArea><placeName><region type="region" nuts="2">Nouvelle-Aquitaine</region><region type="old region" nuts="2">Aquitaine</region><settlement type="city">Bordeaux</settlement></placeName><orgName type="university">Université Bordeaux II</orgName></affiliation></author><author><name sortKey="Coutand, Catherine" sort="Coutand, Catherine" uniqKey="Coutand C" first="Catherine" last="Coutand">Catherine Coutand</name><affiliation wicri:level="3"><nlm:affiliation>¶INRA, UMR 547 PIAF, 234 Avenue du Brézet, F-63100 Clermont-Ferrand, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>¶INRA, UMR 547 PIAF, 234 Avenue du Brézet, F-63100 Clermont-Ferrand</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Auvergne (région administrative)</region><settlement type="city">Clermont-Ferrand</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>∥Clermont Université, Université Blaise Pascal, UMR 547 PIAF, F-63100 Clermont-Ferrand, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>∥Clermont Université, Université Blaise Pascal, UMR 547 PIAF, F-63100 Clermont-Ferrand</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Auvergne (région administrative)</region><settlement type="city">Clermont-Ferrand</settlement></placeName></affiliation></author><author><name sortKey="Plomion, Christophe" sort="Plomion, Christophe" uniqKey="Plomion C" first="Christophe" last="Plomion">Christophe Plomion</name><affiliation wicri:level="3"><nlm:affiliation>†INRA, UMR 1202 BIOGECO, F-33610 Cestas, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>†INRA, UMR 1202 BIOGECO, F-33610 Cestas</wicri:regionArea><placeName><region type="region" nuts="2">Nouvelle-Aquitaine</region><region type="old region" nuts="2">Aquitaine</region><settlement type="city">Cestas</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>‡Univ. Bordeaux, BIOGECO, UMR1202, F-33615 Pessac, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>‡Univ. Bordeaux, BIOGECO, UMR1202, F-33615 Pessac</wicri:regionArea><placeName><region type="region" nuts="2">Nouvelle-Aquitaine</region><region type="old region" nuts="2">Aquitaine</region><settlement type="city">Pessac</settlement></placeName></affiliation></author></analytic><series><title level="j">Journal of proteome research</title><idno type="eISSN">1535-3907</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence (MeSH)</term><term>Cluster Analysis (MeSH)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Gene Ontology (MeSH)</term><term>Gene Regulatory Networks (MeSH)</term><term>Gravitation (MeSH)</term><term>Gravitropism (MeSH)</term><term>Mass Spectrometry (MeSH)</term><term>Molecular Sequence Data (MeSH)</term><term>Peptides (genetics)</term><term>Peptides (metabolism)</term><term>Phosphopeptides (genetics)</term><term>Phosphopeptides (metabolism)</term><term>Phosphoproteins (genetics)</term><term>Phosphoproteins (metabolism)</term><term>Plant Proteins (classification)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (metabolism)</term><term>Populus (genetics)</term><term>Populus (metabolism)</term><term>Proteome (classification)</term><term>Proteome (genetics)</term><term>Proteome (metabolism)</term><term>Proteomics (methods)</term><term>Signal Transduction (genetics)</term><term>Wood (genetics)</term><term>Wood (metabolism)</term><term>Xylem (genetics)</term><term>Xylem (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de regroupements (MeSH)</term><term>Bois (génétique)</term><term>Bois (métabolisme)</term><term>Données de séquences moléculaires (MeSH)</term><term>Gene Ontology (MeSH)</term><term>Gravitation (MeSH)</term><term>Gravitropisme (MeSH)</term><term>Peptides (génétique)</term><term>Peptides (métabolisme)</term><term>Phosphopeptides (génétique)</term><term>Phosphopeptides (métabolisme)</term><term>Phosphoprotéines (génétique)</term><term>Phosphoprotéines (métabolisme)</term><term>Populus (génétique)</term><term>Populus (métabolisme)</term><term>Protéines végétales (classification)</term><term>Protéines végétales (génétique)</term><term>Protéines végétales (métabolisme)</term><term>Protéome (classification)</term><term>Protéome (génétique)</term><term>Protéome (métabolisme)</term><term>Protéomique (méthodes)</term><term>Régulation de l'expression des gènes végétaux (MeSH)</term><term>Réseaux de régulation génique (MeSH)</term><term>Spectrométrie de masse (MeSH)</term><term>Séquence d'acides aminés (MeSH)</term><term>Transduction du signal (génétique)</term><term>Xylème (génétique)</term><term>Xylème (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="classification" xml:lang="en"><term>Plant Proteins</term><term>Proteome</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Peptides</term><term>Phosphopeptides</term><term>Phosphoproteins</term><term>Plant Proteins</term><term>Proteome</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Peptides</term><term>Phosphopeptides</term><term>Phosphoproteins</term><term>Plant Proteins</term><term>Proteome</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="fr"><term>Protéines végétales</term><term>Protéome</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Populus</term><term>Signal Transduction</term><term>Wood</term><term>Xylem</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Bois</term><term>Peptides</term><term>Phosphopeptides</term><term>Phosphoprotéines</term><term>Populus</term><term>Protéines végétales</term><term>Protéome</term><term>Transduction du signal</term><term>Xylème</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Populus</term><term>Wood</term><term>Xylem</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Proteomics</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Bois</term><term>Peptides</term><term>Phosphopeptides</term><term>Phosphoprotéines</term><term>Populus</term><term>Protéines végétales</term><term>Protéome</term><term>Xylème</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Protéomique</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Cluster Analysis</term><term>Gene Expression Regulation, Plant</term><term>Gene Ontology</term><term>Gene Regulatory Networks</term><term>Gravitation</term><term>Gravitropism</term><term>Mass Spectrometry</term><term>Molecular Sequence Data</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de regroupements</term><term>Données de séquences moléculaires</term><term>Gene Ontology</term><term>Gravitation</term><term>Gravitropisme</term><term>Régulation de l'expression des gènes végétaux</term><term>Réseaux de régulation génique</term><term>Spectrométrie de masse</term><term>Séquence d'acides aminés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Trees adjust their growth following forced changes in orientation to re-establish a vertical position. In angiosperms, this adjustment involves the differential regulation of vascular cambial activity between the lower (opposite wood) and upper (tension wood) sides of the leaning stem. We investigated the molecular mechanisms leading to the formation of differential wood types through a quantitative proteomic and phosphoproteomic analysis on poplar subjected to a gravitropic stimulus. We identified and quantified 675 phosphopeptides, corresponding to 468 phosphoproteins, and 3 763 nonphosphorylated peptides, corresponding to 1 155 proteins, in the differentiating xylem of straight-growing trees (control) and trees subjected to a gravitational stimulus during 8 weeks. About 1% of the peptides were specific to a wood type (straight, opposite, or tension wood). Proteins quantified in more than one type of wood were more numerous: a mixed linear model showed 389 phosphopeptides and 556 proteins to differ in abundance between tension wood and opposite wood. Twenty-one percent of the phosphoproteins identified here were described in their phosphorylated form for the first time. Our analyses revealed remarkable developmental molecular plasticity, with wood type-specific phosphorylation events, and highlighted the involvement of different proteins in the biosynthesis of cell wall components during the formation of the three types of wood.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26112267</PMID><DateCompleted><Year>2016</Year><Month>06</Month><Day>06</Day></DateCompleted><DateRevised><Year>2015</Year><Month>08</Month><Day>07</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1535-3907</ISSN><JournalIssue CitedMedium="Internet"><Volume>14</Volume><Issue>8</Issue><PubDate><Year>2015</Year><Month>Aug</Month><Day>07</Day></PubDate></JournalIssue><Title>Journal of proteome research</Title><ISOAbbreviation>J Proteome Res</ISOAbbreviation></Journal><ArticleTitle>Quantitative Proteomic and Phosphoproteomic Approaches for Deciphering the Signaling Pathway for Tension Wood Formation in Poplar.</ArticleTitle><Pagination><MedlinePgn>3188-203</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1021/acs.jproteome.5b00140</ELocationID><Abstract><AbstractText>Trees adjust their growth following forced changes in orientation to re-establish a vertical position. In angiosperms, this adjustment involves the differential regulation of vascular cambial activity between the lower (opposite wood) and upper (tension wood) sides of the leaning stem. We investigated the molecular mechanisms leading to the formation of differential wood types through a quantitative proteomic and phosphoproteomic analysis on poplar subjected to a gravitropic stimulus. We identified and quantified 675 phosphopeptides, corresponding to 468 phosphoproteins, and 3 763 nonphosphorylated peptides, corresponding to 1 155 proteins, in the differentiating xylem of straight-growing trees (control) and trees subjected to a gravitational stimulus during 8 weeks. About 1% of the peptides were specific to a wood type (straight, opposite, or tension wood). Proteins quantified in more than one type of wood were more numerous: a mixed linear model showed 389 phosphopeptides and 556 proteins to differ in abundance between tension wood and opposite wood. Twenty-one percent of the phosphoproteins identified here were described in their phosphorylated form for the first time. Our analyses revealed remarkable developmental molecular plasticity, with wood type-specific phosphorylation events, and highlighted the involvement of different proteins in the biosynthesis of cell wall components during the formation of the three types of wood.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Mauriat</LastName><ForeName>Mélanie</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>†INRA, UMR 1202 BIOGECO, F-33610 Cestas, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>‡Univ. Bordeaux, BIOGECO, UMR1202, F-33615 Pessac, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Leplé</LastName><ForeName>Jean-Charles</ForeName><Initials>JC</Initials><AffiliationInfo><Affiliation>§INRA, UR0588 AGPF, 2163 Avenue de la Pomme de Pin, CS 40001 Ardon, F-45075 Orléans Cedex 2, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Claverol</LastName><ForeName>Stéphane</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>⊥Plateforme Protéome, CGFB, Université Bordeaux Segalen, F-33076 Bordeaux, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bartholomé</LastName><ForeName>Jérôme</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>†INRA, UMR 1202 BIOGECO, F-33610 Cestas, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>‡Univ. Bordeaux, BIOGECO, UMR1202, F-33615 Pessac, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Negroni</LastName><ForeName>Luc</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>⊥Plateforme Protéome, CGFB, Université Bordeaux Segalen, F-33076 Bordeaux, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Richet</LastName><ForeName>Nicolas</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>§INRA, UR0588 AGPF, 2163 Avenue de la Pomme de Pin, CS 40001 Ardon, F-45075 Orléans Cedex 2, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lalanne</LastName><ForeName>Céline</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>†INRA, UMR 1202 BIOGECO, F-33610 Cestas, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>‡Univ. Bordeaux, BIOGECO, UMR1202, F-33615 Pessac, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bonneu</LastName><ForeName>Marc</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>⊥Plateforme Protéome, CGFB, Université Bordeaux Segalen, F-33076 Bordeaux, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Coutand</LastName><ForeName>Catherine</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>¶INRA, UMR 547 PIAF, 234 Avenue du Brézet, F-63100 Clermont-Ferrand, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>∥Clermont Université, Université Blaise Pascal, UMR 547 PIAF, F-63100 Clermont-Ferrand, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Plomion</LastName><ForeName>Christophe</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>†INRA, UMR 1202 BIOGECO, F-33610 Cestas, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>‡Univ. Bordeaux, BIOGECO, UMR1202, F-33615 Pessac, France.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>07</Month><Day>08</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Proteome Res</MedlineTA><NlmUniqueID>101128775</NlmUniqueID><ISSNLinking>1535-3893</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010455">Peptides</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010748">Phosphopeptides</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010750">Phosphoproteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D020543">Proteome</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016000" MajorTopicYN="N">Cluster Analysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D063990" MajorTopicYN="N">Gene Ontology</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D053263" MajorTopicYN="N">Gene Regulatory Networks</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006112" MajorTopicYN="N">Gravitation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018522" MajorTopicYN="N">Gravitropism</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013058" MajorTopicYN="N">Mass Spectrometry</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010455" MajorTopicYN="N">Peptides</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010748" MajorTopicYN="N">Phosphopeptides</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010750" MajorTopicYN="N">Phosphoproteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020543" MajorTopicYN="N">Proteome</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D040901" MajorTopicYN="N">Proteomics</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015398" MajorTopicYN="N">Signal Transduction</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014934" MajorTopicYN="N">Wood</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D052584" MajorTopicYN="N">Xylem</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">cell wall</Keyword><Keyword MajorTopicYN="N">phosphoproteomics</Keyword><Keyword MajorTopicYN="N">poplar</Keyword><Keyword MajorTopicYN="N">proteomics</Keyword><Keyword MajorTopicYN="N">tension wood</Keyword><Keyword MajorTopicYN="N">wood formation</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>6</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>6</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>6</Month><Day>9</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">26112267</ArticleId><ArticleId IdType="doi">10.1021/acs.jproteome.5b00140</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>France</li></country><region><li>Aquitaine</li><li>Auvergne (région administrative)</li><li>Auvergne-Rhône-Alpes</li><li>Centre-Val de Loire</li><li>Nouvelle-Aquitaine</li><li>Région Centre</li></region><settlement><li>Bordeaux</li><li>Cestas</li><li>Clermont-Ferrand</li><li>Orléans</li><li>Pessac</li></settlement><orgName><li>Université Bordeaux II</li></orgName></list><tree><country name="France"><region name="Nouvelle-Aquitaine"><name sortKey="Mauriat, Melanie" sort="Mauriat, Melanie" uniqKey="Mauriat M" first="Mélanie" last="Mauriat">Mélanie Mauriat</name></region><name sortKey="Bartholome, Jerome" sort="Bartholome, Jerome" uniqKey="Bartholome J" first="Jérôme" last="Bartholomé">Jérôme Bartholomé</name><name sortKey="Bartholome, Jerome" sort="Bartholome, Jerome" uniqKey="Bartholome J" first="Jérôme" last="Bartholomé">Jérôme Bartholomé</name><name sortKey="Bonneu, Marc" sort="Bonneu, Marc" uniqKey="Bonneu M" first="Marc" last="Bonneu">Marc Bonneu</name><name sortKey="Claverol, Stephane" sort="Claverol, Stephane" uniqKey="Claverol S" first="Stéphane" last="Claverol">Stéphane Claverol</name><name sortKey="Coutand, Catherine" sort="Coutand, Catherine" uniqKey="Coutand C" first="Catherine" last="Coutand">Catherine Coutand</name><name sortKey="Coutand, Catherine" sort="Coutand, Catherine" uniqKey="Coutand C" first="Catherine" last="Coutand">Catherine Coutand</name><name sortKey="Lalanne, Celine" sort="Lalanne, Celine" uniqKey="Lalanne C" first="Céline" last="Lalanne">Céline Lalanne</name><name sortKey="Lalanne, Celine" sort="Lalanne, Celine" uniqKey="Lalanne C" first="Céline" last="Lalanne">Céline Lalanne</name><name sortKey="Leple, Jean Charles" sort="Leple, Jean Charles" uniqKey="Leple J" first="Jean-Charles" last="Leplé">Jean-Charles Leplé</name><name sortKey="Mauriat, Melanie" sort="Mauriat, Melanie" uniqKey="Mauriat M" first="Mélanie" last="Mauriat">Mélanie Mauriat</name><name sortKey="Negroni, Luc" sort="Negroni, Luc" uniqKey="Negroni L" first="Luc" last="Negroni">Luc Negroni</name><name sortKey="Plomion, Christophe" sort="Plomion, Christophe" uniqKey="Plomion C" first="Christophe" last="Plomion">Christophe Plomion</name><name sortKey="Plomion, Christophe" sort="Plomion, Christophe" uniqKey="Plomion C" first="Christophe" last="Plomion">Christophe Plomion</name><name sortKey="Richet, Nicolas" sort="Richet, Nicolas" uniqKey="Richet N" first="Nicolas" last="Richet">Nicolas Richet</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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